Lucks Lab Engineers Fast RNA Circuitry
Melissa Takahashi and James Chappell of the Lucks Lab, along with students from the first Cold Spring Harbor Synthetic Biology Summer Course, recently published their work on the first measurement of RNA genetic circuitry dynamics in ACS Synthetic Biology. By designing a new experiment to measure the kinetic response of an RNA genetic network to a perturbation, they were able to confirm a long-standing hypothesis that RNA circuitry operates on timescales set by the fast degradation rates of RNAs. They also used these experiments as a rapid prototyping tool to create a new type of RNA genetic network that sequentially stages the expression of proteins in cells. These networks could find important uses for programming the dynamics of gene expression in a variety of application settings, including advanced metabolic engineering.
The article is being published in a special issue of ACS Synthetic Biology dedicated to RNA synthetic biology: http://pubs.acs.org/doi/abs/10.1021/sb400206c
For more information about RNA genetic circuitry, and this work in particular, visit the Lucks Lab website: http://luckslab.org/news/2014/03/our-acs-synbio-paper-on-fast-rna-circuitry-is-out/
For more information about the CSHL Synthetic Biology Summer Course taught by Professor Lucks, Melissa Takahashi, and colleagues, see: http://cshlsynbio.wordpress.com/