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DeLisa lab makes water lovers out of membrane proteins

Thursday, April 9, 2015

Integral membrane proteins are notoriously hard to synthesize and study, which explains why so few have been fully, three-dimensionally characterized with protein crystallography. Using standard recombinant DNA techniques and some novel design principles, Dario Mizrachi has developed a new method for making large quantities of integral membrane proteins simply and inexpensively – all without the use of harsh chemicals, or detergents, typically used today. His method, called “SIMPLEx” (Solubilization of Integral Membrane Proteins with high Levels of Expression), could lead to increased characterization and understanding of this all-important class of proteins, and also could be used to make protein machinery for synthetic biological processes. The work was published online in Nature Communications:

http://www.nature.com/ncomms/2015/150408/ncomms7826/full/ncomms7826.html 

and was also featured in the Cornell Chronicle: 

http://www.news.cornell.edu/stories/2015/04/shield-gives-tricky-proteins-new-identity

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